![]() (a) Raw images, (b) “find edges” filtered images, (c) “median filter” filtered image, (d) “threshold manually selected” segmented image. (B) Montage of time-lapses series of a larva co-cultured with macrophages (Arg1 f/fTie2-Cre) and immune serum in vitro (10 frames, 3 s interval). ![]() Common parts to both masks become null (black) due to the XOR operation. XOR operator on first and last frame masks (c). Mask obtained for the first (b) and last (b′) frame based on a manual drawing. (A) First (a) and last (a′) frame of an ex vivo time-lapse acquisition. Figure S1: Image processing for motility analysis ex vivo and in vitro and quantification of immune-fluorescent staining for Arg1 and F4/80.
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